› 6bpm

X-ray diffraction
2.5Å resolution

The crystal structure of the Ferric-Catecholate import receptor Fiu from K12 E. coli: Closed form (C21)

Released:
DOI: 10.2210/pdb6bpm/pdb
PDB entry 6bpm coloured by chain, front view.
PDB entry 6bpm coloured by chain, side view.
PDB entry 6bpm coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The structure of the bacterial iron-catecholate transporter Fiu suggests that it imports substrates via a two-step mechanism.
Grinter R, Lithgow T
J Biol Chem 294 19523-19534 (2019)
PMID: 31712312

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159995 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catecholate siderophore receptor Fiu Chains: A, C
Molecule details ›
Chains: A, C
Length: 727 amino acids
Theoretical weight: 78.42 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P75780 (Residues: 34-760; Coverage: 100%)
Gene names: JW0790, b0805, fiu, ybiL
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand POG 6 x POG
Ligand BOG 2 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 191.432Å b: 75.668Å c: 136.87Å
α: 90° β: 91.89° γ: 90°
R-values:
R R work R free
0.202 0.199 0.259
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Iron Acquisition Systems of Gram-negative Bacterial Pathogens Define TonB-Dependent Pathways to Novel Antibiotics.
Klebba et al. (2021)
2 more

2 mentions without citation

Molecular Insight into TdfH-Mediated Zinc Piracy from Human Calprotectin by Neisseria gonorrhoeae.
Kammerman et al. (2020)
1 more