› 6big

X-ray diffraction
2.21Å resolution

Crystal structure of cobalt-substituted Synechocystis ACO

Released:
DOI: 10.2210/pdb6big/pdb
PDB entry 6big coloured by chain, front view.
PDB entry 6big coloured by chain, side view.
PDB entry 6big coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
Preparation and characterization of metal-substituted carotenoid cleavage oxygenases.
Sui X, Farquhar ER, Hill HE, von Lintig J, Shi W, Kiser PD
J Biol Inorg Chem 23 887-901 (2018)
PMID: 29946976

Function and Biology Details

Reaction catalysed: 
All-trans-8'-apo-beta-carotenal + O(2) = all-trans-retinal + (2E,4E,6E)-2,6-dimethylocta-2,4,6-trienedial
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apocarotenoid-15,15'-oxygenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 490 amino acids
Theoretical weight: 54.34 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli
UniProt:
  • Canonical: P74334 (Residues: 1-490; Coverage: 100%)
Gene name: sll1541
Sequence domains: Retinal pigment epithelial membrane protein

Ligands and Environments

2 bound ligands:
Ligand CO 4 x CO
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 118.074Å b: 124.531Å c: 202.608Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.229
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Molecular components affecting ocular carotenoid and retinoid homeostasis.
von Lintig et al. (2021)
1 more

1 mention without citation

Preparation and characterization of metal-substituted carotenoid cleavage oxygenases.
Sui et al. (2018)