6bcy

X-ray diffraction
2.3Å resolution

Complex of 14-3-3 theta with an IRSp53 peptide phosphorylated at T360

Released:
Source organism: Homo sapiens
Primary publication:
Mechanism of IRSp53 inhibition by 14-3-3.
Nat Commun 10 483 (2019)
PMID: 30696821

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-150979 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein theta Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 245 amino acids
Theoretical weight: 27.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27348 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAQ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
BAR/IMD domain-containing adapter protein 2 Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 13 amino acids
Theoretical weight: 1.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UQB8 (Residues: 354-366; Coverage: 2%)
Gene name: BAIAP2

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P1
Unit cell:
a: 59.37Å b: 68.63Å c: 84.79Å
α: 72.91° β: 85.59° γ: 64.65°
R-values:
R R work R free
0.193 0.192 0.222
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided