› 6b9x

X-ray diffraction
1.42Å resolution

Crystal structure of Ragulator

Released:
DOI: 10.2210/pdb6b9x/pdb
PDB entry 6b9x coloured by chain, front view.
PDB entry 6b9x coloured by chain, side view.
PDB entry 6b9x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Hybrid Structure of the RagA/C-Ragulator mTORC1 Activation Complex.
Su MY, Morris KL, Kim DJ, Fu Y, Lawrence R, Stjepanovic G, Zoncu R, Hurley JH
Mol Cell 68 835-846.e3 (2017)
PMID: 29107538

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128947 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ragulator complex protein LAMTOR1 Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 17.76 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6IAA8 (Residues: 1-161; Coverage: 100%)
Gene names: C11orf59, LAMTOR1, PDRO, PP7157
Sequence domains: Late endosomal/lysosomal adaptor and MAPK and MTOR activator
Ragulator complex protein LAMTOR2 Chain: B
Molecule details ›
Chain: B
Length: 126 amino acids
Theoretical weight: 13.59 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y2Q5 (Residues: 1-125; Coverage: 100%)
Gene names: HSPC003, LAMTOR2, MAPBPIP, ROBLD3
Sequence domains: Roadblock/LC7 domain
Structure domains: Dynein light chain 2a, cytoplasmic
Ragulator complex protein LAMTOR3 Chain: C
Molecule details ›
Chain: C
Length: 124 amino acids
Theoretical weight: 13.64 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9UHA4 (Residues: 1-124; Coverage: 100%)
Gene names: LAMTOR3, MAP2K1IP1, MAPKSP1, PRO2783
Sequence domains: Mitogen-activated protein kinase kinase 1 interacting
Structure domains: Dynein light chain 2a, cytoplasmic
Ragulator complex protein LAMTOR4 Chain: D
Molecule details ›
Chain: D
Length: 99 amino acids
Theoretical weight: 10.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q0VGL1 (Residues: 1-99; Coverage: 100%)
Gene names: C7orf59, LAMTOR4
Ragulator complex protein LAMTOR5 Chain: E
Molecule details ›
Chain: E
Length: 173 amino acids
Theoretical weight: 18.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43504 (Residues: 1-91; Coverage: 100%)
Gene names: HBXIP, LAMTOR5, XIP
Sequence domains: Ragulator complex protein LAMTOR5
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P61
Unit cell:
a: 168.705Å b: 168.705Å c: 52.325Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.212 0.237
Expression system: Spodoptera frugiperda

Quick links

Citations

16 review citations

mTOR at the nexus of nutrition, growth, ageing and disease.
Liu et al. (2020)
15 more

2 mentions without citation

Structural mechanism of a Rag GTPase activation checkpoint by the lysosomal folliculin complex.
Lawrence et al. (2019)
1 more