› 6b5d

X-ray diffraction
3.1Å resolution

Structural Basis for Katanin Self-Assembly

Released:
DOI: 10.2210/pdb6b5d/pdb
PDB entry 6b5d coloured by chain, front view.
PDB entry 6b5d coloured by chain, side view.
PDB entry 6b5d coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structural basis for disassembly of katanin heterododecamers.
Nithianantham S, McNally FJ, Al-Bassam J
J Biol Chem 293 10590-10605 (2018)
PMID: 29752405

Function and Biology Details

Reaction catalysed: 
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Meiotic spindle formation protein mei-1 Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 34.67 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34808 (Residues: 164-471; Coverage: 65%)
Gene names: T01G9.5, mei-1
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P65
Unit cell:
a: 98.83Å b: 98.83Å c: 75.08Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.211 0.208 0.255
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Microtubule-severing enzymes: From cellular functions to molecular mechanism.
McNally et al. (2018)
2 more

1 mention without citation

Structural basis for disassembly of katanin heterododecamers.
Nithianantham et al. (2018)