› 6aym

X-ray diffraction
1.25Å resolution

Crystal structure of Campylobacter jejuni 5'-methylthioadenosine/S-adenosyl homocysteine nucleosidase (MTAN)

Released:
DOI: 10.2210/pdb6aym/pdb
PDB entry 6aym coloured by chain, front view.
PDB entry 6aym coloured by chain, side view.
PDB entry 6aym coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Transition-State Analogues of Campylobacter jejuni 5'-Methylthioadenosine Nucleosidase.
Ducati RG, Harijan RK, Cameron SA, Tyler PC, Evans GB, Schramm VL
ACS Chem Biol 13 3173-3183 (2018)
PMID: 30339406

Function and Biology Details

Reactions catalysed: 
6-amino-6-deoxyfutalosine + H(2)O = dehypoxanthine futalosine + adenine
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminodeoxyfutalosine nucleosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 26.31 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0PC20 (Residues: 2-229; Coverage: 100%)
Gene names: Cj0117, pfs
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 46.408Å b: 84.62Å c: 124.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.205
Expression system: Escherichia coli

Quick links

Citations

7 citation in other articles

Characterization of 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidases from Borrelia burgdorferi: Antibiotic targets for Lyme disease.
Cornell et al. (2020)
6 more

1 mention without citation

Transition-State Analogues of Campylobacter jejuni 5'-Methylthioadenosine Nucleosidase.
Ducati et al. (2018)