› 6aui

Electron Microscopy
3.3Å resolution

Human ribonucleotide reductase large subunit (alpha) with dATP and CDP

Released:
DOI: 10.2210/pdb6aui/pdb
PDB entry 6aui coloured by chain, front view.
PDB entry 6aui coloured by chain, side view.
PDB entry 6aui coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
3.3-Å resolution cryo-EM structure of human ribonucleotide reductase with substrate and allosteric regulators bound.
Brignole EJ, Tsai KL, Chittuluru J, Li H, Aye Y, Penczek PA, Stubbe J, Drennan CL, Asturias F
Elife 7 (2018)
PMID: 29460780
Related structures: EMD-7006

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase large subunit Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 812 amino acids
Theoretical weight: 92.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23921 (Residues: 1-792; Coverage: 100%)
Gene names: RR1, RRM1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand DTP 12 x DTP
Ligand MG 12 x MG
Ligand CDP 6 x CDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-7006
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Ribonucleotide Reductases: Structure, Chemistry, and Metabolism Suggest New Therapeutic Targets.
Greene et al. (2020)
5 more

4 mentions without citation

The more the merrier: how homo-oligomerization alters the interactome and function of ribonucleotide reductase.
Long et al. (2020)
3 more