› 6aq7

X-ray diffraction
1.83Å resolution

Structure of POM6 FAB fragment complexed with mouse PrPc

Released:
DOI: 10.2210/pdb6aq7/pdb
PDB entry 6aq7 coloured by chain, front view.
PDB entry 6aq7 coloured by chain, side view.
PDB entry 6aq7 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural characterization of POM6 Fab and mouse prion protein complex identifies key regions for prions conformational conversion.
Baral PK, Swayampakula M, Aguzzi A, James MNG
FEBS J 285 1701-1714 (2018)
PMID: 29569342

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230794 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Major prion protein Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 14.48 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04925 (Residues: 127-225; Coverage: 43%)
Gene names: Prn-p, Prnp, Prp
Sequence domains: Prion/Doppel alpha-helical domain
Structure domains: Prion/Doppel protein, beta-ribbon domain
POM6 FAB Heavy CHAIN Chain: H
Molecule details ›
Chain: H
Length: 214 amino acids
Theoretical weight: 23.2 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
POM6 FAB light CHAIN Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.79 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand TCE 1 x TCE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08B1-1
Unit cell:
a: 72.04Å b: 82.42Å c: 103.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.23
Expression systems:
  • Escherichia coli
  • Mus musculus

Quick links

Citations

1 citations in other articles

Development and structural determination of an anti-PrPC aptamer that blocks pathological conformational conversion of prion protein.
Mashima et al. (2020)

4 mentions without citation

Transition of the prion protein from a structured cellular form (PrPC ) to the infectious scrapie agent (PrPSc ).
Baral et al. (2019)
3 more