› 6ao6

X-ray diffraction
2.98Å resolution

Crystal structure of H108A peptidylglycine alpha-hydroxylating monooxygenase (PHM)

Released:
DOI: 10.2210/pdb6ao6/pdb
PDB entry 6ao6 coloured by chain, front view.
PDB entry 6ao6 coloured by chain, side view.
PDB entry 6ao6 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Effects of copper occupancy on the conformational landscape of peptidylglycine α-hydroxylating monooxygenase.
Maheshwari S, Shimokawa C, Rudzka K, Kline CD, Eipper BA, Mains RE, Gabelli SB, Blackburn N, Amzel LM
Commun Biol 1 74 (2018)
PMID: 30271955

Function and Biology Details

Reactions catalysed: 
[Peptide]-glycine + 2 ascorbate + O(2) = [peptide]-(2S)-2-hydroxyglycine + 2 monodehydroascorbate + H(2)O
[Peptide]-(2S)-2-hydroxyglycine = [peptide]-amide + glyoxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidylglycine alpha-amidating monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 34.71 KDa
Source organism: Rattus norvegicus
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P14925 (Residues: 45-356; Coverage: 33%)
Gene name: Pam
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CU 1 x CU
Ligand NI 1 x NI
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 69.123Å b: 69.845Å c: 81.992Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.18 0.261
Expression system: Cricetulus griseus

Quick links

Citations

3 review citations

Copper and the brain noradrenergic system.
Lutsenko et al. (2019)
2 more

3 mentions without citation

Peptidylglycine α-amidating monooxygenase as a therapeutic target or biomarker for human diseases.
Merkler et al. (2022)
2 more