› 6ano

X-ray diffraction
2.61Å resolution

Crystal structure of human FLASH N-terminal domain

Released:
DOI: 10.2210/pdb6ano/pdb
PDB entry 6ano coloured by chain, front view.
PDB entry 6ano coloured by chain, side view.
PDB entry 6ano coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3'-end processing.
Aik WS, Lin MH, Tan D, Tripathy A, Marzluff WF, Dominski Z, Chou CY, Tong L
PLoS One 12 e0186034 (2017)
PMID: 29020104

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CASP8-associated protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 11.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKL3 (Residues: 51-137; Coverage: 4%)
Gene names: CASP8AP2, FLASH, KIAA1315, RIP25

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 66.8Å b: 43.75Å c: 65Å
α: 90° β: 114.51° γ: 90°
R-values:
R R work R free
0.202 0.199 0.252
Expression system: Escherichia coli

Quick links

Citations

1 review citation

U7 deciphered: the mechanism that forms the unusual 3' end of metazoan replication-dependent histone mRNAs.
Dominski et al. (2021)
10 other articles

1 mention without citation

The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3'-end processing.
Aik et al. (2017)