› 6aif

X-ray diffraction
2.3Å resolution

Crystal structure of M120A mutant of O-acetylserine sulfhydrylase from Haemophilus influenzae in complex with high affinity inhibitory peptide from serine acetyl transferase of Salmonella typhimurium

Released:
DOI: 10.2210/pdb6aif/pdb
PDB entry 6aif coloured by chain, front view.
PDB entry 6aif coloured by chain, side view.
PDB entry 6aif coloured by chain, top view.
Source organisms:
Entry authors: Singh RP, Kaushik A

Function and Biology Details

Reactions catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151581 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cysteine synthase Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 37.14 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45040 (Residues: 1-316; Coverage: 100%)
Gene names: HI_1103, cysK
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold
Serine acetyltransferase Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.23 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided
UniProt:
  • Canonical: P29847 (Residues: 264-273; Coverage: 4%)
Gene names: STM3699, cysE

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: I41
Unit cell:
a: 112.16Å b: 112.16Å c: 43.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.214
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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