› 6ai6

X-ray diffraction
2.7Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119555 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9/Csn1 Chain: A
Molecule details ›
Chain: A
Length: 1372 amino acids
Theoretical weight: 159.23 KDa
Source organism: Streptococcus pyogenes serotype M1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99ZW2 (Residues: 1-1368; Coverage: 100%)
Gene names: SPy_1046, cas9, csn1
Sequence domains:
DNA (28-MER) Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.46 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
DNA (8-MER) Chain: D
Molecule details ›
Chain: D
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided
RNA (81-MER) Chain: B
Molecule details ›
Chain: B
Length: 81 nucleotides
Theoretical weight: 26.21 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand K 10 x K
Ligand MG 3 x MG
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 177.377Å b: 68.568Å c: 187.968Å
α: 90° β: 111.51° γ: 90°
R-values:
R R work R free
0.219 0.218 0.244
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

155 review citations

Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors.
Anzalone et al. (2020)
154 more

5 mentions without citation

Engineered CRISPR-Cas9 nuclease with expanded targeting space.
Nishimasu et al. (2018)
4 more