› 6aaw

X-ray diffraction
2Å resolution

Mdm2 in complex with a D amino Acid Containing Stapled Peptide

Released:
DOI: 10.2210/pdb6aaw/pdb
PDB entry 6aaw coloured by chain, front view.
PDB entry 6aaw coloured by chain, side view.
PDB entry 6aaw coloured by chain, top view.
Source organisms:
Entry authors: Brown CJ, Partridge AW

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166405 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase Mdm2 Chain: A
Molecule details ›
Chain: A
Length: 122 amino acids
Theoretical weight: 13.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q00987 (Residues: 6-125; Coverage: 24%)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain
ACE-LEU-THR-PHE-STQ-GLU-TYR-DTR-GLN-LEU-CBA-MK8-SER-ALA-ALA Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.77 KDa
Source organism: Escherichia virus M13
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand DTR 1 x DTR
Ligand MK8 1 x MK8

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: C2
Unit cell:
a: 80.946Å b: 43.378Å c: 34.542Å
α: 90° β: 94.82° γ: 90°
R-values:
R R work R free
0.186 0.184 0.223
Expression systems:
  • Escherichia coli BL21
  • Not provided

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