› 5zzb

X-ray diffraction
2.3Å resolution

LokiProfilin2/Rabbit Actin Complex

Released:
DOI: 10.2210/pdb5zzb/pdb
PDB entry 5zzb coloured by chain, front view.
PDB entry 5zzb coloured by chain, side view.
PDB entry 5zzb coloured by chain, top view.
Source organisms:
Primary publication:
Genomes of Asgard archaea encode profilins that regulate actin.
Akıl C, Robinson RC
Nature 562 439-443 (2018)
PMID: 30283132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101327 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: B, D
Molecule details ›
Chains: B, D
Length: 371 amino acids
Theoretical weight: 41.37 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 7-377; Coverage: 98%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Loki profilin-2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 136 amino acids
Theoretical weight: 14.79 KDa
Source organism: Candidatus Lokiarchaeum sp. GC14_75
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0F8XMN8 (Residues: 1-134; Coverage: 100%)
Gene name: Lokiarch_27400
Sequence domains: Profilin

Ligands and Environments

3 bound ligands:
Ligand LAB 2 x LAB
Ligand ATP 2 x ATP
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 53.85Å b: 123.3Å c: 88.24Å
α: 90° β: 96.9° γ: 90°
R-values:
R R work R free
0.23 0.228 0.274
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Diversity, ecology and evolution of Archaea.
Baker et al. (2020)
14 more

1 mention without citation

Heimdallarchaea encodes profilin with eukaryotic-like actin regulation and polyproline binding.
Survery et al. (2021)