› 5zu9

X-ray diffraction
1.5Å resolution

Crystal structure of MERS-CoV macro domain in complex with ADP

Released:
DOI: 10.2210/pdb5zu9/pdb
PDB entry 5zu9 coloured by chain, front view.
PDB entry 5zu9 coloured by chain, side view.
PDB entry 5zu9 coloured by chain, top view.
Source organism: Middle East respiratory syndrome-related coronavirus
Entry authors: Lin M-H, Cho C-C, Chien C-Y, Hsu C-H

Function and Biology Details

Reaction catalysed: 
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORF1a Chain: A
Molecule details ›
Chain: A
Length: 168 amino acids
Theoretical weight: 17.77 KDa
Source organism: Middle East respiratory syndrome-related coronavirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: T2B9G2 (Residues: 1110-1273; Coverage: 4%)
Sequence domains: Macro domain

Ligands and Environments

1 bound ligand:
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 41.421Å b: 39.423Å c: 45.514Å
α: 90° β: 102.3° γ: 90°
R-values:
R R work R free
0.153 0.15 0.177
Expression system: Escherichia coli BL21(DE3)

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