› 5zsr

X-ray diffraction
2.61Å resolution

NifS from Hydrogenimonas thermophila, soaked with L-cysteine for 8 min

Released:
DOI: 10.2210/pdb5zsr/pdb
PDB entry 5zsr coloured by chain, front view.
PDB entry 5zsr coloured by chain, side view.
PDB entry 5zsr coloured by chain, top view.
Source organism: Hydrogenimonas thermophila
Entry authors: Nakamura R, Fujishiro T, Takahashi Y

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103478 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class V domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 415 amino acids
Theoretical weight: 46.29 KDa
Source organism: Hydrogenimonas thermophila
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1I5NEH3 (Residues: 1-396; Coverage: 100%)
Gene name: SAMN05216234_11013
Sequence domains: Aminotransferase class-V

Ligands and Environments

No bound ligands
1 modified residue:
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P65
Unit cell:
a: 135.9Å b: 135.9Å c: 98.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.194 0.241
Expression system: Escherichia coli

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