› 5zq5

X-ray diffraction
2.49Å resolution

SidE-Ubi

Released:
DOI: 10.2210/pdb5zq5/pdb
PDB entry 5zq5 coloured by chain, front view.
PDB entry 5zq5 coloured by chain, side view.
PDB entry 5zq5 coloured by chain, top view.
Source organisms:
Primary publication:
Structural Insights into Non-canonical Ubiquitination Catalyzed by SidE.
Wang Y, Shi M, Feng H, Zhu Y, Liu S, Gao A, Gao P
Cell 173 1231-1243.e16 (2018)
PMID: 29731171

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143435 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Septation initiation protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 845 amino acids
Theoretical weight: 96.81 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6BBR6 (Residues: 240-1076; Coverage: 55%)
Gene names: C3927_00465, laiD
Sequence domains:
Ubiquitin Chains: B, D
Molecule details ›
Chains: B, D
Length: 79 amino acids
Theoretical weight: 8.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG48 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 34 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 92.672Å b: 129.084Å c: 191.507Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.233
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The role of ubiquitination in tumorigenesis and targeted drug discovery.
Deng et al. (2020)
13 more

2 mentions without citation

Uncovering the Structural Basis of a New Twist in Protein Ubiquitination.
Puvar et al. (2019)
1 more