› 5zjy

X-ray diffraction
1.59Å resolution

Stapled-peptides tailored against initiation of translation

Released:
DOI: 10.2210/pdb5zjy/pdb
PDB entry 5zjy coloured by chain, front view.
PDB entry 5zjy coloured by chain, side view.
PDB entry 5zjy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights reveal a recognition feature for tailoring hydrocarbon stapled-peptides against the eukaryotic translation initiation factor 4E protein.
Lama D, Liberatore AM, Frosi Y, Nakhle J, Tsomaia N, Bashir T, Lane DP, Brown CJ, Verma CS, Auvin S
Chem Sci 10 2489-2500 (2019)
PMID: 30881679

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139169 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 22.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06730 (Residues: 28-217; Coverage: 88%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
LYS-LYS-ARG-TYR-SER-ARG-2JN-GLN-LEU-LEU-2JN-PHE Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.62 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MGP 1 x MGP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P31
Unit cell:
a: 39.8Å b: 39.8Å c: 110.23Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.178 0.216
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Bioinformatics and Biosimulations as Toolbox for Peptides and Peptidomimetics Design: Where Are We?
D'Annessa et al. (2020)
3 more

1 mention without citation

Structural insights reveal a recognition feature for tailoring hydrocarbon stapled-peptides against the eukaryotic translation initiation factor 4E protein.
Lama et al. (2019)