› 5zdo

X-ray diffraction
2.8Å resolution

Crystal Structure Analysis of TtQRS in co-crystallised with ATP

Released:
DOI: 10.2210/pdb5zdo/pdb
PDB entry 5zdo coloured by chain, front view.
PDB entry 5zdo coloured by chain, side view.
PDB entry 5zdo coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structural and functional analysis of Glutaminyl-tRNA synthetase (TtGlnRS) from Thermus thermophilus HB8 and its complexes.
Nachiappan M, Jain V, Sharma A, Yogavel M, Jeyakanthan J
Int J Biol Macromol 120 1379-1386 (2018)
PMID: 30248426

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutamine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 548 amino acids
Theoretical weight: 63.56 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SKU4 (Residues: 1-548; Coverage: 100%)
Gene name: TTHA0549
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 143.868Å b: 39.393Å c: 115.837Å
α: 90° β: 116.82° γ: 90°
R-values:
R R work R free
0.185 0.181 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Conformational heterogeneity in apo and drug-bound structures of Toxoplasma gondii prolyl-tRNA synthetase.
Mishra et al. (2019)
2 more

1 mention without citation

Conformational changes in glutaminyl-tRNA synthetases upon binding of the substrates and analogs using molecular docking and molecular dynamics approaches.
Nachiappan et al. (2020)