5zcs

Electron Microscopy
4.9Å resolution

4.9 Angstrom Cryo-EM structure of human mTOR complex 2

Released:
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of human mTOR complex 2.
Cell Res 28 518-528 (2018)
PMID: 29567957
Related structures: EMD-6913

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-154633 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Serine/threonine-protein kinase mTOR Chains: A, B
Molecule details ›
Chains: A, B
Length: 2549 amino acids
Theoretical weight: 289.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P42345 (Residues: 1-2549; Coverage: 100%)
Gene names: FRAP, FRAP1, FRAP2, MTOR, RAFT1, RAPT1
Sequence domains:
Target of rapamycin complex subunit LST8 Chains: C, D
Molecule details ›
Chains: C, D
Length: 326 amino acids
Theoretical weight: 35.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BVC4 (Residues: 1-326; Coverage: 100%)
Gene names: GBL, LST8, MLST8
Sequence domains: WD domain, G-beta repeat
Rapamycin-insensitive companion of mTOR Chains: E, F
Molecule details ›
Chains: E, F
Length: 1708 amino acids
Theoretical weight: 175.14 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6R327 (Residues: 1-1018; Coverage: 60%)
Gene names: KIAA1999, RICTOR
Sequence domains:
Target of rapamycin complex 2 subunit MAPKAP1 Chains: G, H
Molecule details ›
Chains: G, H
Length: 522 amino acids
Theoretical weight: 55.16 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9BPZ7 (Residues: 141-522; Coverage: 73%)
Gene names: MAPKAP1, MIP1, SIN1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.9Å
Relevant EMDB volumes: EMD-6913
Expression system: Homo sapiens