› 5zab

X-ray diffraction
2.15Å resolution

Crystal structure of cf3-aequorin

Released:
DOI: 10.2210/pdb5zab/pdb
PDB entry 5zab coloured by chain, front view.
PDB entry 5zab coloured by chain, side view.
PDB entry 5zab coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Slow luminescence kinetics of semi-synthetic aequorin: expression, purification and structure determination of cf3-aequorin.
Inouye S, Tomabechi Y, Hosoya T, Sekine SI, Shirouzu M
J Biochem 164 247-255 (2018)
PMID: 29796619

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135947 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aequorin-2 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 198 amino acids
Theoretical weight: 22.54 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P02592 (Residues: 9-196; Coverage: 96%)
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

1 bound ligand:
Ligand 9A3 16 x 9A3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P1
Unit cell:
a: 91.8Å b: 97.55Å c: 121.84Å
α: 77.62° β: 73.06° γ: 75.17°
R-values:
R R work R free
0.196 0.196 0.23
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Chiral deaza-coelenterazine analogs for probing a substrate-binding site in the Ca2+-binding photoprotein aequorin.
Inouye et al. (2021)

1 mention without citation

Crystal structure of semisynthetic obelin-v.
Larionova et al. (2022)