› 5z7d

X-ray diffraction
4.5Å resolution

p204HINab-dsDNA complex structure

Released:
DOI: 10.2210/pdb5z7d/pdb
PDB entry 5z7d coloured by chain, front view.
PDB entry 5z7d coloured by chain, side view.
PDB entry 5z7d coloured by chain, top view.
Source organisms:
Primary publication:
Structural mechanism of DNA recognition by the p204 HIN domain.
Fan X, Jiang J, Zhao D, Chen F, Ma H, Smith P, Unterholzner L, Xiao TS, Jin T
Nucleic Acids Res 49 2959-2972 (2021)
PMID: 33619523

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119485 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Interferon-activable protein 204 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 412 amino acids
Theoretical weight: 46.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DOV2 (Residues: 216-619; Coverage: 65%)
Gene name: Ifi204
Sequence domains: HIN-200/IF120x domain
DNA (5'-D(P*CP*CP*AP*TP*CP*AP*GP*AP*AP*AP*GP*AP*GP*AP*GP*C)-3') Chains: I, K
Molecule details ›
Chains: I, K
Length: 16 nucleotides
Theoretical weight: 4.93 KDa
Source organism: Vaccinia virus
Expression system: Not provided
DNA (5'-D(P*CP*CP*AP*TP*CP*AP*GP*AP*AP*AP*GP*AP*GP*AP*GP*C)-3') Chains: J, L
Molecule details ›
Chains: J, L
Length: 16 nucleotides
Theoretical weight: 4.87 KDa
Source organism: Vaccinia virus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P6522
Unit cell:
a: 101.39Å b: 101.39Å c: 783.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.262 0.26 0.297
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

The Interplay between Viruses and Host DNA Sensors.
Huérfano et al. (2022)
1 more

2 mentions without citation

Activation and Immune Regulation Mechanisms of PYHIN Family During Microbial Infection.
Fan et al. (2021)
1 more