› 5z68

X-ray diffraction
3Å resolution

Structure of the recombination mediator protein RecF-ATP in RecFOR pathway

Released:
DOI: 10.2210/pdb5z68/pdb
PDB entry 5z68 coloured by chain, front view.
PDB entry 5z68 coloured by chain, side view.
PDB entry 5z68 coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Primary publication:
ATP-dependent conformational change in ABC-ATPase RecF serves as a switch in DNA repair.
Tang Q, Liu YP, Shan HH, Tian LF, Zhang JZ, Yan XX
Sci Rep 8 2127 (2018)
PMID: 29391496

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA replication and repair protein RecF Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 373 amino acids
Theoretical weight: 43.39 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis MB4
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8RDL3 (Residues: 1-361; Coverage: 99%)
Gene names: TTE0004, recF
Sequence domains: RecF/RecN/SMC N terminal domain

Ligands and Environments

3 bound ligands:
Ligand ATP 4 x ATP
Ligand IMD 21 x IMD
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U1
Spacegroup: P212121
Unit cell:
a: 108.118Å b: 138.821Å c: 179.567Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.255
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

3 review citations

A Review of the Functional Annotations of Important Genes in the AHPND-Causing pVA1 Plasmid.
Wang et al. (2020)
2 more

3 mentions without citation

ATP-dependent conformational change in ABC-ATPase RecF serves as a switch in DNA repair.
Tang et al. (2018)
2 more