› 5z38

X-ray diffraction
2.29Å resolution

Crystal structure of CsrA bound to CesT

Released:
DOI: 10.2210/pdb5z38/pdb
PDB entry 5z38 coloured by chain, front view.
PDB entry 5z38 coloured by chain, side view.
PDB entry 5z38 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of binding between the global post-transcriptional regulator CsrA and the T3SS chaperone CesT.
Ye F, Yang F, Yu R, Lin X, Qi J, Chen Z, Cao Y, Wei Y, Gao GF, Lu G
Nat Commun 9 1196 (2018)
PMID: 29567971

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159690 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tir chaperone Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 158 amino acids
Theoretical weight: 17.88 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q47015 (Residues: 1-156; Coverage: 100%)
Gene name: cesT
Sequence domains: Tir chaperone protein (CesT) family
Carbon storage regulator Chains: E, G, I, K
Molecule details ›
Chains: E, G, I, K
Length: 49 amino acids
Theoretical weight: 5.21 KDa
Source organism: Escherichia coli O127:H6 str. E2348/69
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69913 (Residues: 1-44; Coverage: 72%)
Gene names: JW2666, b2696, csrA, zfiA
Sequence domains: Global regulator protein family
Carbon storage regulator Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 71 amino acids
Theoretical weight: 7.5 KDa
Source organism: Escherichia coli O127:H6 str. E2348/69
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69913 (Residues: 1-61; Coverage: 100%)
Gene names: JW2666, b2696, csrA, zfiA
Sequence domains: Global regulator protein family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2
Unit cell:
a: 252.75Å b: 52.546Å c: 93.356Å
α: 90° β: 96.73° γ: 90°
R-values:
R R work R free
0.234 0.233 0.251
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Diverse Mechanisms and Circuitry for Global Regulation by the RNA-Binding Protein CsrA.
Pourciau et al. (2020)
5 more

4 mentions without citation

Tyrosine Phosphorylation as a Widespread Regulatory Mechanism in Prokaryotes.
Getz et al. (2019)
3 more