› 5yja

X-ray diffraction
1.65Å resolution

Crystal structure of highly active BTUO mutant P287G without dehydration

Released:
DOI: 10.2210/pdb5yja/pdb
PDB entry 5yja coloured by chain, front view.
PDB entry 5yja coloured by chain, side view.
PDB entry 5yja coloured by chain, top view.
Source organism: Bacillus sp. TB-90
Entry authors: Hibi T, Itoh T, Nishiya Y

Function and Biology Details

Reactions catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uric acid degradation bifunctional protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 323 amino acids
Theoretical weight: 37.07 KDa
Source organism: Bacillus sp. TB-90
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q45697 (Residues: 172-494; Coverage: 64%)
Gene name: uao
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

5 bound ligands:
Ligand AZA 4 x AZA
Ligand OXY 4 x OXY
Ligand SO4 2 x SO4
Ligand K 2 x K
Ligand MXE 2 x MXE
1 modified residue:
Ligand OCS 4 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21212
Unit cell:
a: 133.017Å b: 144.252Å c: 70.272Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.187
Expression system: Escherichia coli DH5[alpha]

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