› 5y8g

X-ray diffraction
2.01Å resolution

Mycobacterium tuberculosis 3-Hydroxyisobutyrate Dehydrogenase (MtHIBADH)

Released:
DOI: 10.2210/pdb5y8g/pdb
PDB entry 5y8g coloured by chain, front view.
PDB entry 5y8g coloured by chain, side view.
PDB entry 5y8g coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structure, interactions and action of Mycobacterium tuberculosis 3-hydroxyisobutyric acid dehydrogenase.
Srikalaivani R, Singh A, Vijayan M, Surolia A
Biochem J 475 2457-2471 (2018)
PMID: 29959185

Function and Biology Details

Reaction catalysed: 
3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161906 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable 3-hydroxyisobutyrate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 295 amino acids
Theoretical weight: 29.84 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WNY5 (Residues: 1-294; Coverage: 100%)
Gene names: MTV041.25c, Rv0751c, mmsB
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand AKR 2 x AKR
Ligand GOL 3 x GOL
Ligand 9ON 2 x 9ON
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P65
Unit cell:
a: 128.79Å b: 128.79Å c: 70.35Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.146 0.144 0.185
Expression system: Escherichia coli BL21(DE3)

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