› 5xxr

X-ray diffraction
2.65Å resolution

Crystal structure of selenomethionine labelled RIBT from Bacillus subtilis

Released:
DOI: 10.2210/pdb5xxr/pdb
PDB entry 5xxr coloured by chain, front view.
PDB entry 5xxr coloured by chain, side view.
PDB entry 5xxr coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural characterization of ribT from Bacillus subtilis reveals it as a GCN5-related N-acetyltransferase.
Srivastava R, Kaur A, Sharma C, Karthikeyan S
J Struct Biol 202 70-81 (2018)
PMID: 29241954

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148123 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein RibT Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 15.9 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17622 (Residues: 1-124; Coverage: 100%)
Gene names: BSU23240, ribT
Sequence domains: Acetyltransferase (GNAT) family

Ligands and Environments

1 bound ligand:
Ligand COA 2 x COA
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 54.572Å b: 57.55Å c: 73.088Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.276
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Production of Vitamin B2 (Riboflavin) by Microorganisms: An Overview.
Averianova et al. (2020)
1 more