› 5xox

X-ray diffraction
3Å resolution

Crystal structure of tRNA(His) guanylyltranserase from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb5xox/pdb
PDB entry 5xox coloured by chain, front view.
PDB entry 5xox coloured by chain, side view.
PDB entry 5xox coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Crystal structure of tRNAHis guanylyltransferase from Saccharomyces cerevisiae.
Lee K, Lee EH, Son J, Hwang KY
Biochem Biophys Res Commun 490 400-405 (2017)
PMID: 28623126

Function and Biology Details

Reaction catalysed: 
(1a) p-tRNA(His) + ATP = A-Ppp-tRNA(His) + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA(His) guanylyltransferase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 243 amino acids
Theoretical weight: 28.62 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P53215 (Residues: 1-237; Coverage: 100%)
Gene names: THG1, YGR024C
Sequence domains:
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand GTP 5 x GTP
Ligand MG 10 x MG
Ligand PO4 5 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: I41
Unit cell:
a: 164.703Å b: 164.703Å c: 275.891Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.274
Expression system: Escherichia coli

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Citations

1 review citation

The Role of 3' to 5' Reverse RNA Polymerization in tRNA Fidelity and Repair.
Chen et al. (2019)
3 other articles