› 5xam

X-ray diffraction
4Å resolution

Crystal structure of SecDF in I form at 4 A resolution

Released:
DOI: 10.2210/pdb5xam/pdb
PDB entry 5xam coloured by chain, front view.
PDB entry 5xam coloured by chain, side view.
PDB entry 5xam coloured by chain, top view.
Source organism: Deinococcus radiodurans R1
Primary publication:
Tunnel Formation Inferred from the I-Form Structures of the Proton-Driven Protein Secretion Motor SecDF.
Furukawa A, Yoshikaie K, Mori T, Mori H, Morimoto YV, Sugano Y, Iwaki S, Minamino T, Sugita Y, Tanaka Y, Tsukazaki T
Cell Rep 19 895-901 (2017)
PMID: 28467902

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multifunctional fusion protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 750 amino acids
Theoretical weight: 80.65 KDa
Source organism: Deinococcus radiodurans R1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RTE3 (Residues: 28-768; Coverage: 97%)
Gene names: DR_1822, secD, secF
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P21
Unit cell:
a: 93.32Å b: 62.26Å c: 181.71Å
α: 90° β: 101.71° γ: 90°
R-values:
R R work R free
0.34 0.333 0.402
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Alav et al. (2021)
8 more

2 mentions without citation

Structure and Dynamics of the Central Lipid Pool and Proteins of the Bacterial Holo-Translocon.
Martin et al. (2019)
1 more