› 5x37

Solution NMR

Solution structure of the Family 1 carbohydrate-binding module with mannosylated Ser14

Released:
DOI: 10.2210/pdb5x37/pdb
PDB entry 5x37 coloured by chain, ensemble of 20 models, front view.
PDB entry 5x37 coloured by chain, ensemble of 20 models, side view.
PDB entry 5x37 coloured by chain, ensemble of 20 models, top view.
Source organism: Trichoderma reesei
Primary publication:
Structural Insight into the Stabilizing Effect of O-Glycosylation.
Chaffey PK, Guan X, Chen C, Ruan Y, Wang X, Tran AH, Koelsch TN, Cui Q, Feng Y, Tan Z
Biochemistry 56 2897-2906 (2017)
PMID: 28494147

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158661 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exoglucanase 1 Chain: A
Molecule details ›
Chain: A
Length: 36 amino acids
Theoretical weight: 3.75 KDa
Source organism: Trichoderma reesei
Expression system: Not provided
UniProt:
  • Canonical: P62694 (Residues: 478-513; Coverage: 7%)
Gene name: cbh1
Sequence domains: Fungal cellulose binding domain

Ligands and Environments

1 bound ligand:
Ligand MAN 1 x MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

4 review citations

Synthesis and biological roles of O-glycans in insects.
Li et al. (2020)
3 more