› 5wzk

X-ray diffraction
2.8Å resolution

Structure of APUM23-deletion-of-insert-region-GGAAUUGACGG

Released:
DOI: 10.2210/pdb5wzk/pdb
PDB entry 5wzk coloured by chain, front view.
PDB entry 5wzk coloured by chain, side view.
PDB entry 5wzk coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the specific recognition of 18S rRNA by APUM23.
Bao H, Wang N, Wang C, Jiang Y, Liu J, Xu L, Wu J, Shi Y
Nucleic Acids Res 45 12005-12014 (2017)
PMID: 29036323

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189914 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Pumilio homolog 23 Chain: A
Molecule details ›
Chain: A
Length: 546 amino acids
Theoretical weight: 61.7 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9C552 (Residues: 85-655; Coverage: 71%)
Gene names: APUM23, At1g72320, T10D10.21, T9N14.7
Sequence domains: Pumilio-family RNA binding repeat
RNA (5'-R(*GP*GP*AP*AP*UP*UP*GP*AP*CP*GP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.59 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P65
Unit cell:
a: 189.06Å b: 189.06Å c: 45.456Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.18 0.225
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

Quick links

Citations

1 review citation

Post-transcriptional Regulatory Functions of Mammalian Pumilio Proteins.
Goldstrohm et al. (2018)
4 other articles

1 mention without citation

Structural basis for the specific recognition of 18S rRNA by APUM23.
Bao et al. (2017)