› 5ww3

X-ray diffraction
2.05Å resolution

Crystal structure of the second DNA-Binding protein under starvation from Mycobacterium smegmatis soaked with iron in the ratio of 24 iron atoms per dodecamer

Released:
DOI: 10.2210/pdb5ww3/pdb
PDB entry 5ww3 coloured by chain, front view.
PDB entry 5ww3 coloured by chain, side view.
PDB entry 5ww3 coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Flexible aspartates propel iron to the ferroxidation sites along pathways stabilized by a conserved arginine in Dps proteins from Mycobacterium smegmatis.
Williams SM, Chatterji D
Metallomics 9 685-698 (2017)
PMID: 28418062

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferritin/DPS protein domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 161 amino acids
Theoretical weight: 17.85 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QXB7 (Residues: 1-161; Coverage: 100%)
Gene name: MSMEG_3242
Sequence domains: Ferritin-like domain
Structure domains: Ferritin

Ligands and Environments

4 bound ligands:
Ligand FE 4 x FE
Ligand FE2 6 x FE2
Ligand MG 2 x MG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: R32
Unit cell:
a: 90.17Å b: 90.17Å c: 420.411Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.164 0.199
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Bacterial iron detoxification at the molecular level.
Bradley et al. (2020)
4 more