5wie

X-ray diffraction
3.3Å resolution

Crystal structure of a Kv1.2-2.1 chimera K+ channel V406W mutant in an inactivated state

Released:
Source organisms:
Primary publication:
Crystal structure of an inactivated mutant mammalian voltage-gated K+ channel.
Nat Struct Mol Biol 24 857-865 (2017)
PMID: 28846092

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-242886 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Voltage-gated potassium channel subunit beta-2 Chains: A, G
Molecule details ›
Chains: A, G
Length: 333 amino acids
Theoretical weight: 37.35 KDa
Source organism: Rattus norvegicus
Expression system: Pichia
UniProt:
  • Canonical: P62483 (Residues: 35-367; Coverage: 91%)
Gene names: Ckbeta2, Kcnab2, Kcnb3
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain
Potassium voltage-gated channel subfamily A member 2 Chains: B, H
Molecule details ›
Chains: B, H
Length: 532 amino acids
Theoretical weight: 60.46 KDa
Source organism: Mus musculus
Expression system: Pichia
UniProt:
  • Canonical: P63141 (Residues: 1-14, 16-30, 33-206, 208-265, 280-499; Coverage: 96%)
Gene name: Kcna2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P4212
Unit cell:
a: 143.124Å b: 143.124Å c: 284.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.243
Expression system: Pichia