› 5wc1

X-ray diffraction
3.3Å resolution

katanin AAA ATPase domain

Released:
DOI: 10.2210/pdb5wc1/pdb
PDB entry 5wc1 coloured by chain, front view.
PDB entry 5wc1 coloured by chain, side view.
PDB entry 5wc1 coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Katanin spiral and ring structures shed light on power stroke for microtubule severing.
Zehr E, Szyk A, Piszczek G, Szczesna E, Zuo X, Roll-Mecak A
Nat Struct Mol Biol 24 717-725 (2017)
PMID: 28783150

Function and Biology Details

Reaction catalysed: 
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Meiotic spindle formation protein mei-1 Chain: A
Molecule details ›
Chain: A
Length: 472 amino acids
Theoretical weight: 51.8 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P34808 (Residues: 1-472; Coverage: 100%)
Gene names: T01G9.5, mei-1
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P65
Unit cell:
a: 99.363Å b: 99.363Å c: 76.581Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.248 0.308
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Structure and Function of the 26S Proteasome.
Bard et al. (2018)
13 more

3 mentions without citation

Microtubule-severing enzymes: From cellular functions to molecular mechanism.
McNally et al. (2018)
2 more