› 5wbc

X-ray diffraction
1.72Å resolution

Designed Artificial Cupredoxins - WT

Released:
DOI: 10.2210/pdb5wbc/pdb
PDB entry 5wbc coloured by chain, front view.
PDB entry 5wbc coloured by chain, side view.
PDB entry 5wbc coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Modular Artificial Cupredoxins.
Mann SI, Heinisch T, Weitz AC, Hendrich MP, Ward TR, Borovik AS
J Am Chem Soc 138 9073-6 (2016)
PMID: 27385206

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 16.57 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 38-183; Coverage: 92%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

3 bound ligands:
Ligand CU6 1 x CU6
Ligand GOL 1 x GOL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I4122
Unit cell:
a: 57.58Å b: 57.58Å c: 183.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.134 0.168
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Computational approaches for de novo design and redesign of metal-binding sites on proteins.
Akcapinar et al. (2017)
2 more

4 mentions without citation

Artificial Metalloenzymes Based on the Biotin-Streptavidin Technology: Enzymatic Cascades and Directed Evolution.
Liang et al. (2019)
3 more