› 5w0s

Electron Microscopy
3.5Å resolution

GroEL using cryoEM

Released:
DOI: 10.2210/pdb5w0s/pdb
PDB entry 5w0s coloured by chain, ensemble of 4 models, front view.
PDB entry 5w0s coloured by chain, ensemble of 4 models, side view.
PDB entry 5w0s coloured by chain, ensemble of 4 models, top view.
Source organism: Escherichia coli
Primary publication:
Subunit conformational variation within individual GroEL oligomers resolved by Cryo-EM.
Roh SH, Hryc CF, Jeong HH, Fei X, Jakana J, Lorimer GH, Chiu W
Proc Natl Acad Sci U S A 114 8259-8264 (2017)
PMID: 28710336
Related structures: EMD-8750

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperonin GroEL Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 524 amino acids
Theoretical weight: 55.15 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6F5 (Residues: 2-525; Coverage: 96%)
Gene names: JW4103, b4143, groEL, groL, mopA
Sequence domains: TCP-1/cpn60 chaperonin family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-8750
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Chaperonin of Group I: Oligomeric Spectrum and Biochemical and Biological Implications.
Vilasi et al. (2017)
13 more

14 mentions without citation

Subunit conformational variation within individual GroEL oligomers resolved by Cryo-EM.
Roh et al. (2017)
13 more