Function and Biology Details
Reactions catalysed:
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
L-cysteine + 2-oxoglutarate = 2-oxo-3-sulfanylpropanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
Aspartate aminotransferase, cytoplasmic (preferred)
PDBe Complex ID:
PDB-CPX-132569 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aspartate aminotransferase, cytoplasmic
Molecule details ›
Chain: A
Length: 412 amino acids
Theoretical weight: 46.39 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
Sequence domains: Aminotransferase class I and II
Structure domains: Type I PLP-dependent aspartate aminotransferase-like (Major domain)
Length: 412 amino acids
Theoretical weight: 46.39 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
- Canonical:
P00503 (Residues: 2-413; Coverage: 100%)
Sequence domains: Aminotransferase class I and II
Structure domains: Type I PLP-dependent aspartate aminotransferase-like (Major domain)
Aspartate aminotransferase, cytoplasmic
Molecule details ›
Chain: B
Length: 412 amino acids
Theoretical weight: 46.62 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
Sequence domains: Aminotransferase class I and II
Structure domains: Type I PLP-dependent aspartate aminotransferase-like (Major domain)
Length: 412 amino acids
Theoretical weight: 46.62 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
- Canonical: P00503 (Residues: 2-413; Coverage: 100%)
Sequence domains: Aminotransferase class I and II
Structure domains: Type I PLP-dependent aspartate aminotransferase-like (Major domain)
Ligands and Environments
Experiments and Validation Details
Experimental Method:
Neutron Diffraction, X-ray diffraction
Spacegroup:
P212121
Unit cell:
a: 55.532Å
b: 124.986Å
c: 130.444Å
α: 90°
β: 90°
γ: 90°
R-values:
R
R work
R free
0.197
0.234
0.221
Expression system: Escherichia coli
