› 5vfb

X-ray diffraction
1.36Å resolution

1.36 Angstrom Resolution Crystal Structure of Malate Synthase G from Pseudomonas aeruginosa in Complex with Glycolic Acid.

Released:
DOI: 10.2210/pdb5vfb/pdb
PDB entry 5vfb coloured by chain, front view.
PDB entry 5vfb coloured by chain, side view.
PDB entry 5vfb coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Minasov G, Shuvalova L, Dubrovska I, Kiryukhina O, Grimshaw S, Kwon K, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191436 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate synthase G Chains: A, B
Molecule details ›
Chains: A, B
Length: 728 amino acids
Theoretical weight: 79.03 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I636 (Residues: 1-725; Coverage: 100%)
Gene names: PA0482, glcB
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NI 2 x NI
Ligand CL 10 x CL
Ligand GOA 2 x GOA
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 63.385Å b: 111.712Å c: 96.954Å
α: 90° β: 100.42° γ: 90°
R-values:
R R work R free
0.145 0.143 0.168
Expression system: Escherichia coli BL21(DE3)

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