› 5v45

X-ray diffraction
1.91Å resolution

Crystal structure of the F270M, K291M, L318M mutant of SR1 domain of human sacsin

Released:
DOI: 10.2210/pdb5v45/pdb
PDB entry 5v45 coloured by chain, front view.
PDB entry 5v45 coloured by chain, side view.
PDB entry 5v45 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations.
Ménade M, Kozlov G, Trempe JF, Pande H, Shenker S, Wickremasinghe S, Li X, Hojjat H, Dicaire MJ, Brais B, McPherson PS, Wong MJH, Young JC, Gehring K
J Biol Chem 293 12832-12842 (2018)
PMID: 29945973

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sacsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 28.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9NZJ4 (Residues: 89-336; Coverage: 5%)
Gene names: DNAJC29, KIAA0730, SACS

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P1
Unit cell:
a: 41.679Å b: 41.36Å c: 74.106Å
α: 99.55° β: 95.26° γ: 107.5°
R-values:
R R work R free
0.188 0.186 0.224
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Genetic updates on paroxysmal dyskinesias.
Liao et al. (2021)
2 more

1 mention without citation

Structures of ubiquitin-like (Ubl) and Hsp90-like domains of sacsin provide insight into pathological mutations.
Ménade et al. (2018)