› 5v2x

X-ray diffraction
1.85Å resolution

Ethylene forming enzyme in complex with manganese and 2-oxoglutarate

Released:
DOI: 10.2210/pdb5v2x/pdb
PDB entry 5v2x coloured by chain, front view.
PDB entry 5v2x coloured by chain, side view.
PDB entry 5v2x coloured by chain, top view.
Source organism: Pseudomonas savastanoi pv. phaseolicola
Primary publication:
Structures and Mechanisms of the Non-Heme Fe(II)- and 2-Oxoglutarate-Dependent Ethylene-Forming Enzyme: Substrate Binding Creates a Twist.
Martinez S, Fellner M, Herr CQ, Ritchie A, Hu J, Hausinger RP
J Am Chem Soc 139 11980-11988 (2017)
PMID: 28780854

Function and Biology Details

Reactions catalysed: 
2-oxoglutarate + O(2) = ethylene + 3 CO(2) + H(2)O
(1a) 2-oxoglutarate + L-arginine + O(2) = succinate + CO(2) + 5-hydroxy-L-arginine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152192 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-oxoglutarate-dependent ethylene/succinate-forming enzyme Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 39.72 KDa
Source organism: Pseudomonas savastanoi pv. phaseolicola
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P32021 (Residues: 1-350; Coverage: 100%)
Gene name: efe
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand AKG 4 x AKG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 74.411Å b: 97.546Å c: 102.385Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.201
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Amazing Diversity in Biochemical Roles of Fe(II)/2-Oxoglutarate Oxygenases.
Herr et al. (2018)
5 more

3 mentions without citation

Recent examples of α-ketoglutarate-dependent mononuclear non-haem iron enzymes in natural product biosyntheses.
Gao et al. (2018)
2 more