5usb

X-ray diffraction
1.62Å resolution

Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssRNA/ssDNA chimera (rGGTTACGGT)

Released:
Source organism: Schizosaccharomyces pombe
Primary publication:
Discrimination against RNA Backbones by a ssDNA Binding Protein.
Structure 26 722-733.e2 (2018)
PMID: 29681468

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-113192 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA/RNA hybrid molecule
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 139 amino acids
Theoretical weight: 16.72 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O13988 (Residues: 199-337; Coverage: 25%)
Gene names: SPAC26H5.06, pot1
Sequence domains: ssDNA-binding domain of telomere protection protein
Structure domains: Nucleic acid-binding proteins
G1R_9mer DNA/RNA (5'-R(*G)-D(P*GP*TP*TP*AP*CP*GP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 9 nucleotides
Theoretical weight: 2.8 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 41.29Å b: 58.01Å c: 65.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.176 0.203
Expression systems:
  • Escherichia coli BL21
  • Not provided