› 5udp

X-ray diffraction
1.35Å resolution

High resolution x-ray crystal structure of synthetic insulin lispro

Released:
DOI: 10.2210/pdb5udp/pdb
PDB entry 5udp coloured by chain, front view.
PDB entry 5udp coloured by chain, side view.
PDB entry 5udp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Scope and Limitations of Fmoc Chemistry SPPS-Based Approaches to the Total Synthesis of Insulin Lispro via Ester Insulin.
Dhayalan B, Mandal K, Rege N, Weiss MA, Eitel SH, Meier T, Schoenleber RO, Kent SB
Chemistry 23 1709-1716 (2017)
PMID: 27905149

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134224 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

4 bound ligands:
Ligand IPH 6 x IPH
Ligand ZN 2 x ZN
Ligand CL 2 x CL
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 46.644Å b: 61.47Å c: 59.148Å
α: 90° β: 110.6° γ: 90°
R-values:
R R work R free
0.161 0.16 0.194
Expression system: Not provided

Quick links

Citations

2 review citations

Novel protein science enabled by total chemical synthesis.
Kent SBH. (2019)
1 more