› 5ubx

X-ray diffraction
2.7Å resolution

Crystal structure of a mutant mIgG2b Fc heterodimer in complex with Protein A peptide analog Z34C

Released:
DOI: 10.2210/pdb5ubx/pdb
PDB entry 5ubx coloured by chain, front view.
PDB entry 5ubx coloured by chain, side view.
PDB entry 5ubx coloured by chain, top view.
Source organisms:
Primary publication:
Modulation of protein A binding allows single-step purification of mouse bispecific antibodies that retain FcRn binding.
Zwolak A, Armstrong AA, Tam SH, Pardinas JR, Goulet DR, Zheng S, Brosnan K, Emmell E, Luo J, Gilliland GL, Chiu ML
MAbs 9 1306-1316
PMID: 28898162

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134929 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Immunoglobulin heavy constant gamma 2B Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 25.88 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P01867 (Residues: 108-334; Coverage: 56%)
Gene names: Igh-3, Ighg2b
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 2B Chain: B
Molecule details ›
Chain: B
Length: 236 amino acids
Theoretical weight: 26.63 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
UniProt:
  • Canonical: P01867 (Residues: 108-334; Coverage: 56%)
Gene names: Igh-3, Ighg2b
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Peptide analog of B-domain from Protein A - Z34C Chain: Z
Molecule details ›
Chain: Z
Length: 34 amino acids
Theoretical weight: 4.19 KDa
Source organism: Staphylococcus aureus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, FUC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P43212
Unit cell:
a: 101.49Å b: 101.49Å c: 135.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.209 0.257
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

2 review citations

Antibody Structure and Function: The Basis for Engineering Therapeutics.
Chiu et al. (2019)
1 more

1 mention without citation

Modulation of protein A binding allows single-step purification of mouse bispecific antibodies that retain FcRn binding.
Zwolak et al. (2017)