› 5u9b

Solution NMR

Solution structure of the zinc fingers 1 and 2 of MBNL1 in complex with human cardiac troponin T pre-mRNA

Released:
DOI: 10.2210/pdb5u9b/pdb
PDB entry 5u9b coloured by chain, ensemble of 20 models, front view.
PDB entry 5u9b coloured by chain, ensemble of 20 models, side view.
PDB entry 5u9b coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis for Interaction of the Tandem Zinc Finger Domains of Human Muscleblind with Cognate RNA from Human Cardiac Troponin T.
Park S, Phukan PD, Zeeb M, Martinez-Yamout MA, Dyson HJ, Wright PE
Biochemistry 56 4154-4168 (2017)
PMID: 28718627

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192544 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Muscleblind-like protein 1 Chain: A
Molecule details ›
Chain: A
Length: 92 amino acids
Theoretical weight: 10.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR56 (Residues: 1-92; Coverage: 24%)
Gene names: EXP, KIAA0428, MBNL, MBNL1
Sequence domains: RNA-binding, Nab2-type zinc finger
Structure domains: Ribosomal Protein S8; Chain: A, domain 1
RNA (5'-R(P*GP*UP*CP*UP*CP*GP*CP*UP*UP*UP*UP*CP*CP*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.62 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 59%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

Short Tandem Repeat Expansions and RNA-Mediated Pathogenesis in Myotonic Dystrophy.
Sznajder et al. (2019)
6 more

2 mentions without citation

Structural Basis for Interaction of the Tandem Zinc Finger Domains of Human Muscleblind with Cognate RNA from Human Cardiac Troponin T.
Park et al. (2017)
1 more