› 5u7n

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF A CHIMERIC CUA DOMAIN (SUBUNIT II) OF CYTOCHROME BA3 FROM THERMUS THERMOPHILUS WITH THE AMICYANIN LOOP

Released:
DOI: 10.2210/pdb5u7n/pdb
PDB entry 5u7n coloured by chain, front view.
PDB entry 5u7n coloured by chain, side view.
PDB entry 5u7n coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Engineering a bifunctional copper site in the cupredoxin fold by loop-directed mutagenesis.
Espinoza-Cara A, Zitare U, Alvarez-Paggi D, Klinke S, Otero LH, Murgida DH, Vila AJ
Chem Sci 9 6692-6702 (2018)
PMID: 30310603

Function and Biology Details

Reaction catalysed: 
4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c oxidase subunit 2 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5SJ80 (Residues: 44-149, 160-168; Coverage: 69%)
Gene names: TTHA1134, cbaB, ctaC
Sequence domains: Cytochrome C oxidase subunit II, periplasmic domain
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU 8 x CU
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER D8 QUEST
Spacegroup: P212121
Unit cell:
a: 94.352Å b: 100.701Å c: 101.103Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.226
Expression system: Escherichia coli BL21

Quick links

Citations

1 citations in other articles

Stepwise nitrosylation of the nonheme iron site in an engineered azurin and a molecular basis for nitric oxide signaling mediated by nonheme iron proteins.
Tian et al. (2021)

2 mentions without citation

Engineering a bifunctional copper site in the cupredoxin fold by loop-directed mutagenesis.
Espinoza-Cara et al. (2018)
1 more