› 5ttd

X-ray diffraction
2Å resolution

Minor pilin FctB from S. pyogenes with engineered intramolecular isopeptide bond

Released:
DOI: 10.2210/pdb5ttd/pdb
PDB entry 5ttd coloured by chain, front view.
PDB entry 5ttd coloured by chain, side view.
PDB entry 5ttd coloured by chain, top view.
Source organisms:
Primary publication:
Engineering a Lys-Asn isopeptide bond into an immunoglobulin-like protein domain enhances its stability.
Kwon H, Young PG, Squire CJ, Baker EN
Sci Rep 7 42753 (2017)
PMID: 28202898

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112374 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Streptococcal pilin isopeptide linker domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 55.11 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 33-392; Coverage: 97%)
  • Canonical: D2KPJ6 (Residues: 1-122; Coverage: 88%)
Gene names: JW3994, b4034, fctB, malE
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand FMT 4 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2221
Unit cell:
a: 180.359Å b: 192.093Å c: 68.204Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Genetics, Structure, and Function of Group A Streptococcal Pili.
Nakata et al. (2021)
3 other articles

1 mention without citation

Engineering a Lys-Asn isopeptide bond into an immunoglobulin-like protein domain enhances its stability.
Kwon et al. (2017)