› 5tmb

X-ray diffraction
2.34Å resolution

Crystal structure of Os79 from O. sativa in complex with UDP.

Released:
DOI: 10.2210/pdb5tmb/pdb
PDB entry 5tmb coloured by chain, front view.
PDB entry 5tmb coloured by chain, side view.
PDB entry 5tmb coloured by chain, top view.
Source organism: Oryza sativa Japonica Group
Primary publication:
Crystal Structure of Os79 (Os04g0206600) from Oryza sativa: A UDP-glucosyltransferase Involved in the Detoxification of Deoxynivalenol.
Wetterhorn KM, Newmister SA, Caniza RK, Busman M, McCormick SP, Berthiller F, Adam G, Rayment I
Biochemistry 55 6175-6186 (2016)
PMID: 27715009

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-glycosyltransferase 79 Chain: A
Molecule details ›
Chain: A
Length: 467 amino acids
Theoretical weight: 51.14 KDa
Source organism: Oryza sativa Japonica Group
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q7XT97 (Residues: 1-466; Coverage: 100%)
Gene names: LOC_Os04g12970, OS79, OSJNBa0052O21.15, Os04g0206600, OsJ_13826, UGT79
Sequence domains: UDP-glucoronosyl and UDP-glucosyl transferase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 105.502Å b: 105.502Å c: 100.187Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.195 0.192 0.244
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

5 review citations

Strategies and Methodologies for Developing Microbial Detoxification Systems to Mitigate Mycotoxins.
Zhu et al. (2017)
4 more

2 mentions without citation

Structure-function and engineering of plant UDP-glycosyltransferase.
Wang et al. (2023)
1 more