› 5t6j

X-ray diffraction
1.75Å resolution

Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly

Released:
DOI: 10.2210/pdb5t6j/pdb
PDB entry 5t6j coloured by chain, front view.
PDB entry 5t6j coloured by chain, side view.
PDB entry 5t6j coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.
Dimitrova YN, Jenni S, Valverde R, Khin Y, Harrison SC
Cell 167 1014-1027.e12 (2016)
PMID: 27881300

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-153999 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Kinetochore protein SPC24 Chain: A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 6.94 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04477 (Residues: 155-213; Coverage: 28%)
Gene names: SPC24, YM9718.16C, YMR117C
Sequence domains: Spc24 subunit of Ndc80
Structure domains: Double Stranded RNA Binding Domain
Kinetochore protein SPC25 Chain: B
Molecule details ›
Chain: B
Length: 92 amino acids
Theoretical weight: 10.03 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40014 (Residues: 133-221; Coverage: 40%)
Gene names: SPC25, YER018C
Structure domains: Chromosome segregation protein Spc25
Kinetochore-associated protein DSN1 Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.45 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P40568 (Residues: 560-572; Coverage: 2%)
Gene names: DSN1, YIB10W, YIR010W

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: I4122
Unit cell:
a: 85.889Å b: 85.889Å c: 95.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.242
Expression system: Escherichia coli

Quick links

Citations

16 review citations

A Molecular View of Kinetochore Assembly and Function.
Musacchio et al. (2017)
15 more

1 mention without citation

Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.
Dimitrova et al. (2016)