› 5t0g

Electron Microscopy
4.4Å resolution

Structural basis for dynamic regulation of the human 26S proteasome

Released:
DOI: 10.2210/pdb5t0g/pdb
PDB entry 5t0g coloured by chain, front view.
PDB entry 5t0g coloured by chain, side view.
PDB entry 5t0g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for dynamic regulation of the human 26S proteasome.
Chen S, Wu J, Lu Y, Ma YB, Lee BH, Yu Z, Ouyang Q, Finley DJ, Kirschner MW, Mao Y
Proc Natl Acad Sci U S A 113 12991-12996 (2016)
PMID: 27791164
Related structures: EMD-8334

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 32-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126174 (preferred)
Entry contents:
32 distinct polypeptide molecules
Macromolecules (32 distinct):
Proteasome subunit alpha type-6 Chain: G
Molecule details ›
Chain: G
Length: 245 amino acids
Theoretical weight: 27.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60900 (Residues: 2-246; Coverage: 100%)
Gene names: PROS27, PSMA6
Sequence domains:
Proteasome subunit alpha type-2 Chain: H
Molecule details ›
Chain: H
Length: 233 amino acids
Theoretical weight: 25.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25787 (Residues: 2-234; Coverage: 100%)
Gene names: HC3, PSC3, PSMA2
Sequence domains:
Proteasome subunit alpha type-4 Chain: I
Molecule details ›
Chain: I
Length: 260 amino acids
Theoretical weight: 29.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25789 (Residues: 2-261; Coverage: 100%)
Gene names: HC9, PSC9, PSMA4
Sequence domains:
Proteasome subunit alpha type-7 Chain: J
Molecule details ›
Chain: J
Length: 247 amino acids
Theoretical weight: 27.8 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O14818 (Residues: 2-248; Coverage: 100%)
Gene names: HSPC, PSMA7
Sequence domains:
Proteasome subunit alpha type-5 Chain: K
Molecule details ›
Chain: K
Length: 240 amino acids
Theoretical weight: 26.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28066 (Residues: 2-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Proteasome subunit alpha type-1 Chain: L
Molecule details ›
Chain: L
Length: 268 amino acids
Theoretical weight: 30.15 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25786 (Residues: 2-263; Coverage: 100%)
Gene names: HC2, NU, PROS30, PSC2, PSMA1
Sequence domains:
Proteasome subunit alpha type-3 Chain: M
Molecule details ›
Chain: M
Length: 254 amino acids
Theoretical weight: 28.34 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P25788 (Residues: 2-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Proteasome subunit beta type-6 Chain: N
Molecule details ›
Chain: N
Length: 238 amino acids
Theoretical weight: 25.25 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28072 (Residues: 2-239; Coverage: 100%)
Gene names: LMPY, PSMB6, Y
Sequence domains: Proteasome subunit
Proteasome subunit beta type-7 Chain: O
Molecule details ›
Chain: O
Length: 276 amino acids
Theoretical weight: 29.87 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99436 (Residues: 2-277; Coverage: 100%)
Gene names: PSMB7, Z
Sequence domains:
Proteasome subunit beta type-3 Chain: P
Molecule details ›
Chain: P
Length: 204 amino acids
Theoretical weight: 22.84 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49720 (Residues: 2-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Proteasome subunit beta type-2 Chain: Q
Molecule details ›
Chain: Q
Length: 201 amino acids
Theoretical weight: 22.86 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P49721 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Proteasome subunit beta type-5 Chain: R
Molecule details ›
Chain: R
Length: 262 amino acids
Theoretical weight: 28.38 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28074 (Residues: 2-263; Coverage: 100%)
Gene names: LMPX, MB1, PSMB5, X
Sequence domains: Proteasome subunit
Proteasome subunit beta type-1 Chain: S
Molecule details ›
Chain: S
Length: 240 amino acids
Theoretical weight: 26.39 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P20618 (Residues: 2-241; Coverage: 100%)
Gene names: PSC5, PSMB1
Sequence domains: Proteasome subunit
Proteasome subunit beta type-4 Chain: T
Molecule details ›
Chain: T
Length: 263 amino acids
Theoretical weight: 29.1 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P28070 (Residues: 2-264; Coverage: 100%)
Gene names: PROS26, PSMB4
Sequence domains: Proteasome subunit
26S proteasome regulatory subunit 7 Chain: A
Molecule details ›
Chain: A
Length: 433 amino acids
Theoretical weight: 48.7 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P35998 (Residues: 1-433; Coverage: 100%)
Gene names: MSS1, PSMC2
Sequence domains:
26S proteasome regulatory subunit 4 Chain: B
Molecule details ›
Chain: B
Length: 440 amino acids
Theoretical weight: 49.26 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62191 (Residues: 1-440; Coverage: 100%)
Gene name: PSMC1
Sequence domains:
26S proteasome regulatory subunit 6B Chain: D
Molecule details ›
Chain: D
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P43686 (Residues: 1-418; Coverage: 100%)
Gene names: MIP224, PSMC4, TBP7
Sequence domains:
26S proteasome regulatory subunit 10B Chain: E
Molecule details ›
Chain: E
Length: 403 amino acids
Theoretical weight: 45.87 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62333 (Residues: 1-389; Coverage: 100%)
Gene names: PSMC6, SUG2
Sequence domains:
26S proteasome regulatory subunit 6A Chain: F
Molecule details ›
Chain: F
Length: 439 amino acids
Theoretical weight: 49.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P17980 (Residues: 1-439; Coverage: 100%)
Gene names: PSMC3, TBP1
Sequence domains:
26S proteasome regulatory subunit 8 Chain: C
Molecule details ›
Chain: C
Length: 398 amino acids
Theoretical weight: 44.85 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P62195 (Residues: 9-406; Coverage: 98%)
Gene names: PSMC5, SUG1
Sequence domains:
26S proteasome non-ATPase regulatory subunit 1 Chain: U
Molecule details ›
Chain: U
Length: 953 amino acids
Theoretical weight: 105.96 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q99460 (Residues: 1-953; Coverage: 100%)
Gene name: PSMD1
Sequence domains:
26S proteasome non-ATPase regulatory subunit 3 Chain: V
Molecule details ›
Chain: V
Length: 533 amino acids
Theoretical weight: 60.94 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O43242 (Residues: 2-534; Coverage: 100%)
Gene name: PSMD3
Sequence domains:
26S proteasome non-ATPase regulatory subunit 12 Chain: W
Molecule details ›
Chain: W
Length: 456 amino acids
Theoretical weight: 52.98 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00232 (Residues: 1-456; Coverage: 100%)
Gene name: PSMD12
Sequence domains:
26S proteasome non-ATPase regulatory subunit 11 Chain: X
Molecule details ›
Chain: X
Length: 422 amino acids
Theoretical weight: 47.53 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00231 (Residues: 1-422; Coverage: 100%)
Gene name: PSMD11
Sequence domains:
26S proteasome non-ATPase regulatory subunit 6 Chain: Y
Molecule details ›
Chain: Y
Length: 389 amino acids
Theoretical weight: 45.59 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q15008 (Residues: 1-389; Coverage: 100%)
Gene names: KIAA0107, PFAAP4, PSMD6
Sequence domains:
26S proteasome non-ATPase regulatory subunit 7 Chain: Z
Molecule details ›
Chain: Z
Length: 324 amino acids
Theoretical weight: 37.09 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P51665 (Residues: 1-324; Coverage: 100%)
Gene names: MOV34L, PSMD7
Sequence domains:
26S proteasome non-ATPase regulatory subunit 13 Chain: a
Molecule details ›
Chain: a
Length: 376 amino acids
Theoretical weight: 43 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UNM6 (Residues: 1-376; Coverage: 100%)
Gene name: PSMD13
Sequence domains: PCI domain
26S proteasome non-ATPase regulatory subunit 4 Chain: b
Molecule details ›
Chain: b
Length: 377 amino acids
Theoretical weight: 40.78 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P55036 (Residues: 1-377; Coverage: 100%)
Gene names: MCB1, PSMD4
Sequence domains:
26S proteasome non-ATPase regulatory subunit 14 Chain: c
Molecule details ›
Chain: c
Length: 309 amino acids
Theoretical weight: 34.49 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: O00487 (Residues: 2-310; Coverage: 100%)
Gene names: POH1, PSMD14
Sequence domains: JAB1/Mov34/MPN/PAD-1 ubiquitin protease
26S proteasome non-ATPase regulatory subunit 8 Chain: d
Molecule details ›
Chain: d
Length: 349 amino acids
Theoretical weight: 39.54 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P48556 (Residues: 2-350; Coverage: 100%)
Gene name: PSMD8
Sequence domains: CSN8/PSMD8/EIF3K family
26S proteasome complex subunit SEM1 Chain: e
Molecule details ›
Chain: e
Length: 70 amino acids
Theoretical weight: 8.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P60896 (Residues: 1-70; Coverage: 100%)
Gene names: C7orf76, DSS1, SEM1, SHFDG1, SHFM1
Sequence domains: DSS1/SEM1 family
26S proteasome non-ATPase regulatory subunit 2 Chain: f
Molecule details ›
Chain: f
Length: 749 amino acids
Theoretical weight: 82.28 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13200 (Residues: 162-908; Coverage: 82%)
Gene names: PSMD2, TRAP2
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 6 x ATP
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-8334

Quick links

Citations

33 review citations

The Logic of the 26S Proteasome.
Collins et al. (2017)
32 more

7 mentions without citation

Proteasome Structure and Assembly.
Budenholzer et al. (2017)
6 more