› 5sy7

X-ray diffraction
4.2Å resolution

Crystal Structure of the Heterodimeric NPAS3-ARNT Complex with HRE DNA

Released:
DOI: 10.2210/pdb5sy7/pdb
PDB entry 5sy7 coloured by chain, front view.
PDB entry 5sy7 coloured by chain, side view.
PDB entry 5sy7 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
NPAS1-ARNT and NPAS3-ARNT crystal structures implicate the bHLH-PAS family as multi-ligand binding transcription factors.
Wu D, Su X, Potluri N, Kim Y, Rastinejad F
Elife 5 (2016)
PMID: 27782878

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119124 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Aryl hydrocarbon receptor nuclear translocator Chain: A
Molecule details ›
Chain: A
Length: 384 amino acids
Theoretical weight: 43.44 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53762 (Residues: 81-464; Coverage: 49%)
Gene name: Arnt
Sequence domains:
Neuronal PAS domain-containing protein 3 Chain: B
Molecule details ›
Chain: B
Length: 410 amino acids
Theoretical weight: 46.23 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9QZQ0 (Residues: 56-455; Coverage: 43%)
Gene names: Mop6, Npas3
Sequence domains:
DNA (5'-D(*GP*GP*CP*TP*GP*CP*GP*TP*AP*CP*GP*TP*GP*CP*GP*GP*GP*TP*CP*GP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.53 KDa
DNA (5'-D(*CP*AP*CP*GP*AP*CP*CP*CP*GP*CP*AP*CP*GP*TP*AP*CP*GP*CP*AP*GP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.36 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43
Unit cell:
a: 64.831Å b: 64.831Å c: 249.102Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.276 0.272 0.361
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Development and Therapeutic Potential of Small-Molecule Modulators of Circadian Systems.
Chen et al. (2018)
9 more

12 mentions without citation

bHLH-PAS Proteins: Their Structure and Intrinsic Disorder.
Kolonko et al. (2019)
11 more